TOP
Sanger sequencing
Sequencing Sample Type | Sample Request |
Plasmids | Concentration > 50 ng/µl, volume > 5µl/reaction, large fragment plasmids (> 10kb) please indicate length on sequencing sheet |
Purified PCR product | Concentration >10ng/µl, volume >5µl/reaction, electrophoretically detected bands are single and correctly sized |
PCR products with non-uniform bands | Concentration > 10ng/µl, volume > 20µl PCR product with single band: concentration > 10ng/µl, volume > 20µl. If the fragment size is < 200bp, it is recommended to sequence after cloning; take 2µl of the sample for electrophoresis before delivery to ensure that the target bands are clearly visible. |
Bacterial solution | Place 50 µl (not less than 30 µl) of fresh overnight culture or glycerol in a 1.5 ml centrifuge tube, clearly labeled with the sample number. |
Plate colony | Please label and number the selected colonies to be tested and use a cushion to prevent breakage during shipping. |