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Sanger sequencing

Sequencing Sample Type Sample Request
Plasmids Concentration > 50 ng/µl, volume > 5µl/reaction, large fragment plasmids (> 10kb) please indicate length on sequencing sheet
Purified PCR product Concentration >10ng/µl, volume >5µl/reaction, electrophoretically detected bands are single and correctly sized
PCR products with non-uniform bands Concentration > 10ng/µl, volume > 20µl PCR product with single band: concentration > 10ng/µl, volume > 20µl.
If the fragment size is < 200bp, it is recommended to sequence after cloning; take 2µl of the sample for electrophoresis before delivery to ensure that the target bands are clearly visible.
Bacterial solution Place 50 µl (not less than 30 µl) of fresh overnight culture or glycerol in a 1.5 ml centrifuge tube, clearly labeled with the sample number.
Plate colony Please label and number the selected colonies to be tested and use a cushion to prevent breakage during shipping.

 

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