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Gene point mutation cell line construction

Point mutation is a technique to replace an exogenous mutation site with a specific site in the genome by homologous terminal recombination (HDR), so as to achieve a targeted mutation of a gene and obtain stable inheritance. Currently, point mutation is mainly realized by the CRISPR/Cas9 system combined with the Donor sequence into the cell at the same time, through the intracellular homologous recombination repair mechanism in the Cas cutting site to introduce the Donor sequence containing the point mutation site of the gene sequence.
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Service Advantages

Low off-target efficiency using dual sgRNA/RNP delivery

Low off-target efficiency using dual sgRNA/RNP delivery

01/03
Phenotypically stable for long term transmission

Phenotypically stable for long term transmission

02/03
Pure/Heterozygous monoclonal clones are selected according to customer needs;

Pure/Heterozygous monoclonal clones are selected according to customer needs;

03/03

Service Period

Cell Classification Service cycle
Active cells 10-16 weeks
Low Difficulty Cells
Moderately Difficult Cells 12-16 weeks
Difficulty Cells 12-18 weeks

 

Service Process

Cell line validation

Cell line validation

cell transfection

cell transfection

cell expansion

cell expansion

Pool efficiency validation

Pool efficiency validation

Screening and validation

Screening and validation

Cellular validation

Cellular validation

Delivery Results

If you have any other requirements, please send an email to order@gentlegen.com,We will serve you as soon as possible.

Mutationally pure monoclonal strain 1

control cell line

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Inquiry and Ordering

Inquiry and Ordering

Directly log in the “GBS Online Ordering System”, automatically get a quote and place an order; download the corresponding service order form in the “Download” area, send an e-mail, we will provide you with a quote as soon as possible.

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Ordering Information

Ordering Information

Email:marketing@gentlegen.com Tel:0512-67998818 ext. 8888

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