Sanger Sequencing Service

Sanger sequencing service

In the field of life sciences, GentleGen has been providing top-notch Sanger sequencing services to researchers in many professional organizations. These organizations include academic institutions, pharmaceutical companies, GLP companies, biotechnology companies focusing on biological exploration, and governmental organizations undertaking important scientific research tasks.
Sanger sequencing, as the most accurate sequencing method, has important applications in scientific research. It is mainly used in routine cloning testing to provide accurate data support for cloning experiments; it plays a key role in single-gene testing to help scientists explore the mysteries of genes; and it also demonstrates its unique value in a small number of individual-level studies, such as small-throughput screening. Because of its high accuracy and reliability, Sanger sequencing is regarded as the “gold standard of sequencing”.

Service Type

Sanger sequencing

strain identification

SNP detection and mutation analysis

Microsatellite (SSR/STR) analysis

Monoclonal Antibody Sequencing

Cell line analysis

TA clone sequencing

Type of service	Sample type	lead time	Sample Delivery Criteria	Delivery standards
Sanger sequencing	Plasmids, PCR products	1-2 working days	Concentration > 50 ng/µl, volume > 5µl/reaction, large fragment plasmids (> 10kb) please indicate length on sequencing sheet
	Purified PCR product	1-2 working days	Concentration >10ng/µl, volume >5µl/reaction, electrophoretically detected bands are single and correctly sized
	Unpurified PCR product	1-2 working days	PCR product without a single band: concentration > 10ng/µl, volume > 20µl PCR product with a single band: concentration > 10ng/µl, volume > 5µl/reaction If the fragment size is < 200bp, it is recommended to sequence it after cloning; take 2µl of sample for electrophoresis to ensure that the target bands are clearly visible before sending the sample.
	bacterial solution	1-2 working days	Please fill a 1.5 ml centrifuge tube with 50 µl (not less than 30 µl) of fresh or glycerolized bacteria from the overnight culture, clearly labeled with the sample number.
	plate colony	1-2 working days	Plate colonies: Please circle and number the colonies to be measured and use a cushion to prevent breakage during transportation.
strain identification	genomic DNA	<24 samples, 7-9 business days	Concentration ≥ 50 ng/μl, total volume ≥ 10 μl; no significant degradation	PCR product electropherogram, PCR product sequencing results blast results, lab report
SNP detection and mutation analysis	genomic DNA	Number ≤ 15; 5 working days; each additional 15; 3 additional working days	Sample dry weight ≥0.5g, number of cultured cells ≥5*106	PCR amplification + sequencing
SNP detection and mutation analysis	nucleic acid sample	Number of amplifications ≤96; 4-5 working days; for each additional 96 amplifications, increase by 3 working days.	Volume ≥10µl; Concentration ≥20ng/µl; No significant degradation	PCR amplification + sequencing
Microsatellite (SSR/STR) analysis	Fluorescent PCR products	Number of running boards ≤ 5 boards, 5 working days	Volume ≥ 5µl; Concentration ≥ 20ng/µl (per electrophoresis reaction)	Running board results (.fsa file, PDF version) Results analysis file (Excel sheet)
Microsatellite (SSR/STR) analysis	genomic DNA	Amplification number ≤ 5 plates, 7-10 working days	Volume ≥ 10µl; Concentration ≥ 20ng/µl
Monoclonal Antibody Sequencing	genomic DNA	Heavy and light chain variable area sequencing: 7 business days within 24 samples	Freshly collected hybridoma cell sediment: 5×106 cells or more	Sequencing results (.ab1 & .seq) Constructed plasmid samples (optional) Antibody sequencing report
Monoclonal Antibody Sequencing	nucleic acid sample	Heavy and light chain variable + constant region sequencing: 10 working days within 24 samples	Total RNA: Concentration of not less than 100 ng/μl, volume of 10 μl or more, total RNA of 2 μg or more, clear bands without obvious degradation, RIN value of 7 or more.
Cell line analysis	Cellular STR identification	5-7 working days	Genomic DNA: concentration ≥ 50 ng/μL, total volume ≥ 10 μL; and no significant degradation Cell suspension or precipitate: cell number ≥ 10⁶	Sequencing results (.ab1 & .seq)
	Mycoplasma detection	3-5 working days	Cell supernatant: cultured for 48h-72h, with cell density of at least 80% and volume ≥1ml.
	cell identification	5-7 working days	Provision of genomic DNA, cell suspension or precipitate for cellular STR identification Provision of cell supernatant for mycoplasma detection
TA clone sequencing	<800bp	working days	Sample name, fragment size, whether it was purified or not, whether the product had an A-tail or not	Sequencing results (.ab1 & .seq)
TA clone sequencing	800-2000bp	7个工作日		Sequencing results (.ab1 & .seq)

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Service Process

Template Preparation

Quantitative dilution

Sequencing spiking

On-line testing

Automatic interpretation of results

Service Advantages

One-stop-shop for customer-related needs

Covering a comprehensive range of Sanger sequencing and peripheral services in the market, we can provide one-stop service for customers' related needs.

01/05

Fully automated production line

The production cycle is 20% shorter than the traditional manual production line, and the fully automated production line, from plasmid to sequencing results delivery, can effectively avoid personnel errors, production efficiency, quality instability and other problems.

02/05

Optimal sequencing protocols for complex structures

Sequencing protocols for complex templates, with sequencing results recognized by customers.

03/05

higher quality

Continuous effective read length is guaranteed at 800 bp, up to 1000 bp.

04/05

Free universal primers

Universal primers fit most standard vectors

05/05

Application Area

Gene Therapy

Antibody Drugs

Question & Answer

Why did the sequencing fill out the sequencing pass 3k and the sequencing result was only about 1k?

Normal a sequencing results to ensure the accuracy of about 800bp, long fragment sequencing requires multiple reaction sequencing results splicing in order to pass, such as the order sequencing requirements fill in the pass we will be based on the results of the sequencing results have been designed to extend to the pass and after the pass to send you the results of the splicing, such as the process of passing the test in the process of the problem will be timely feedback to you.

Why is the sequencing result positive when the synthesized primer information is filled in and reverse sequencing is told?

Sequencing is based on the primer sequence provided by the teacher to synthesize and sequencing, sequencing can not control the primer direction are based on the primer direction extension sequencing, such as synthesis of primers need to reverse sequencing needs to be the teacher to carry out their own reverse complementary, or to send an order to inform the sequence of the need to carry out the reverse complementary we will arrange for you to reverse complementary and then synthesize and sequencing.

Why must fragment lengths be filled in for PCR samples?

Because PCR sample sequencing we need to identify whether the band size meets your expectations, if not, it is likely that the amplified band is not your target band, resulting in sequencing does not meet expectations, and identification of the existence of multiple bands we can also be based on the length of the fragments provided by you to cut the gel recovery of your requested band for sequencing.

Service&Support

Inquiry and Ordering

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Ordering Information

inbox:order@gentlegen.com Tel:0512-67998818 ext. 8888

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