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Mammalian Cell Expression Services

Mammalian Cell Expression Services

Mammalian cell expression systems for recombinant proteins can undergo post-translational modifications and express recombinant proteins close to the human conformation. This feature makes mammalian cell expression systems the most widely used in the development and production of recombinant protein drugs, especially therapeutic recombinant proteins. Mammalian cell expression systems are an important part of genetic engineering and pharmaceutical research.

GentleGen has a one-stop service platform from gene synthesis to recombinant protein expression and purification, which provides codon optimization to construct high expression vectors, and at the same time selects high secretion signal peptides to improve recombinant protein expression. Our recombinant protein production service uses HEK293 or CHO cells as host cells to produce high-quality recombinant proteins through the high-expression transient platform.
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Service Advantages

High expression vector: select high secretion signal peptide and construct high expression vector independently.

High expression vector: select high secretion signal peptide and construct high expression vector independently.

01/03
High expression system: mammalian cell (CHO/HEK293) high expression system

High expression system: mammalian cell (CHO/HEK293) high expression system

02/03
Rapid gene synthesis platform: clone construction in just 72 hours

Rapid gene synthesis platform: clone construction in just 72 hours

03/03

Service Period

Codon optimization and expression vector construction, plasmid duplication and plasmid validation

Service Description Service Description Service Cycle Delivery Content
Gene synthesis and plasmid duplication 10-15 days Report on the analysis of small-trial expression results
SDS-PAGE analysis results
WB results
express oneself in a small way Transfection of mammalian cells One-step affinity purification QC assay
Amplified expression (1-50L) Amplified expression, protein purification, QC assay 10-15 days Purified proteins(SDS-PAGE>90%,internal poison <1EU/mg) Shipment Reports、SEC-HPLC

 

Service Process

Gene synthesis

Gene synthesis

Plasmid Big Draw

Plasmid Big Draw

Transient expression

Transient expression

Protein purification

Protein purification

Quality control

Quality control

Cold chain shipping

Cold chain shipping

Delivery Results

If you have other requests, send an e-mail to order@gentlegen.com,We will be at your service as soon as possible.

Analysis of results report

Purified proteins

Shipment Reports

SEC-HPLC

Service&Support

Inquiry and Ordering

Inquiry and Ordering

Directly log in the “GBS Online Ordering System”, automatically get a quote and place an order; download the corresponding service order form in the “Download” area, send an e-mail, we will provide you with a quote as soon as possible.

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Product Brochure Download、Order form

Ordering Information

Ordering Information

Email:marketing@gentlegen.com Tel:0512-67998818 ext. 8888

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