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Eukaryotic/prokaryotic transcriptome sequencing

Eukaryotic/prokaryotic transcriptome sequencing can comprehensively and rapidly obtain almost all gene transcripts of a specific cell or tissue of a species in a certain state, which can be used for studying gene structure and function, alternative splicing, and predicting new transcripts. For eukaryotes, with a reference genome available, sequencing based on measurements can simultaneously analyze the sequence and structural information of transcripts such as SNPs and alternative splicing with high precision; In addition, it has unique advantages in detecting low abundance transcripts and discovering new transcripts. The vast majority of mRNA in prokaryotes does not have polyA tails, so rRNA removal can be used for library construction. Chain specific sequencing can be used to perform high-throughput sequencing of both mRNA and ncRNA in prokaryotes.
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Service Advantages

Flexible project customization and bioinformatics services

Flexible project customization and bioinformatics services

01/05
Less restrictions on starting materials and strict quality control

Less restrictions on starting materials and strict quality control

02/05
Sequencing data quality: Q20>90%, Q30>85%

Sequencing data quality: Q20>90%, Q30>85%

03/05
Library construction: Ability to build libraries compatible with special samples such as tissues, cells, FFPE, plasma, and exosomes

Library construction: Ability to build libraries compatible with special samples such as tissues, cells, FFPE, plasma, and exosomes

04/05
Quickly deliver project results

Quickly deliver project results

05/05

Service Process

experimental design

experimental design

Sample collection

Sample collection

nucleic acid extraction

nucleic acid extraction

library construction

library construction

High throughput sequencing

High throughput sequencing

Bioinformatics analysis

Bioinformatics analysis

Delivery Results

If you have any other requirements, please send an email to order@gentlegen.com,We will serve you as soon as possible.

Comparison and Counts Calculation

Differential gene expression analysis

SNV+InDel

Differential gene GO/KEGG function

New transcriptome analysis

Service&Support

Inquiry and ordering

Inquiry and ordering

Directly log in to GentleGen GBS Online Ordering System,Automatically obtain quotes and place orders; stay“Data Download”Download the corresponding service subscription form and send an email. We will provide you with a quotation as soon as possible.

Data Download

Data Download

Product manual download、order form

ordering information

ordering information

Email:marketing@gentlegen.com Phone: 0512-67998818, ext. 8888

Sample Preparation

Total RNA and animal and plant tissues, cell samples, blood samples, microbial samples, FFRE samples

Total RNA and animal and plant tissues, cell samples, blood samples, microbial samples, FFRE samples

Eukaryotes: Total sample size: ≥ 1.5 μ g Sample concentration: ≥ 50 ng/ul

Eukaryotes: Total sample size: ≥ 1.5 μ g Sample concentration: ≥ 50 ng/ul

Prokaryote: Total sample size: ≥ 2 μ g Sample concentration: ≥ 100 ng/ul

Prokaryote: Total sample size: ≥ 2 μ g Sample concentration: ≥ 100 ng/ul

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