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Knockout cell line construction

Knock out (KO) is an important regulatory method to achieve gene loss of function, which can completely eliminate the expression of the target gene, so that its protein is not expressed at all or its function is completely lost, and it can better observe the changes of the cell phenotype.CRISPR/Cas9 generally achieves the knock out of genes in two ways: code-shift mutation and large fragment knockout, both of which can be provided by Junliang Biologicals for customers. CRISPR/Cas9 can be utilized in two ways: shift mutation and blockbuster knockout. In addition, JUNJI BIO can provide one-on-one customized services to meet customers' requirements at all levels.
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Service Advantages

Guaranteed service with delivery of pure monoclones (optional heterozygous monoclones)

Guaranteed service with delivery of pure monoclones (optional heterozygous monoclones)

01/03
Flexible shipping method: frozen or live cells can be shipped.

Flexible shipping method: frozen or live cells can be shipped.

02/03
Strict testing and quality control to ensure sterility and mycoplasma-free contamination; cell viability testing to ensure delivery quality

Strict testing and quality control to ensure sterility and mycoplasma-free contamination; cell viability testing to ensure delivery quality

03/03

Service Period

Cell classification Completion cycle (working days)
Hundreds of Spot Cells 1-2 Weeks
Hundreds of special-priced cells 5-10 Weeks
Low Difficulty Cells 6-10 weeks
Moderately Difficult Cells 8-14 Weeks
Difficult Cells 8-14 weeks

Service Process

Cell line validation

Cell line validation

cell transfection

cell transfection

ko cell expansion

ko cell expansion

Screening and Cultivation

Screening and Cultivation

ko verification

ko verification

Delivery Results

If you have any other requirements, please send an email to marketing@gentlegen.com,We will serve you as soon as possible.

Knockout heterozygous monoclonal strain 1

control cell line

Sequencing results

DNA level validation

Lab Report

Service&Support

Inquiry and Ordering

Inquiry and Ordering

Directly log in the “GBS Online Ordering System”, automatically get a quote and place an order; download the corresponding service order form in the “Download” area, send an e-mail, we will provide you with a quote as soon as possible.

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Ordering Information

Ordering Information

Email:marketing@gentlegen.com Tel:0512-67998818 ext. 8888

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