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Knock-in cell line construction

Knock in (KI) is a gene editing technique in which an exogenous gene is inserted into the genome by homologous end recombination to stabilize its expression in the cell. Currently, gene knock-in is mainly realized by transfecting CRISPR/Cas9 system and DNA template into cells at the same time, and after DNA double-strand breaks at the target site caused by gRNA and Cas9 complexes, the cells use Donor as a template to perform homologous recombination repair (HDR) to realize the targeted fragment knock-in.
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Service Advantages

Low off-target efficiency using dual sgRNA/RNP delivery

Low off-target efficiency using dual sgRNA/RNP delivery

01/03
Flexible shipping method: frozen or live cells can be shipped.

Flexible shipping method: frozen or live cells can be shipped.

02/03
Extensive cell bank to provide diversified chassis cells for customers

Extensive cell bank to provide diversified chassis cells for customers

03/03

Service Period

cell classification Completion cycle (working days)
Low Difficulty Cells 6-10 weeks
Moderately difficult cells 8-14 weeks
difficulty cell 8-14 weeks

Service Process

Cell line validation

Cell line validation

cell transfection

cell transfection

KI cell expansion

KI cell expansion

Pool efficiency validation

Pool efficiency validation

Screening and Cultivation

Screening and Cultivation

KI Verification

KI Verification

Delivery Results

If you have any other requirements, please send an email to order@gentlegen.com,We will serve you as soon as possible.

Knock-in heterozygous monoclonal strain 1

control cell line

Sequencing results

DNA level validation

Lab Report

Service&Support

Inquiry and Ordering

Inquiry and Ordering

Directly log in the “GBS Online Ordering System”, automatically get a quote and place an order; download the corresponding service order form in the “Download” area, send an e-mail, we will provide you with a quote as soon as possible.

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Product Brochure Download、Order form

Ordering Information

Ordering Information

Email:marketing@gentlegen.com Tel:0512-67998818 ext. 8888

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