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sgRNA synthesis service

sgRNA synthesis service

Single guide RNA (sgRNA) is crucial in CRISPR/Cas9 technology. The sgRNA is modified from crRNA and tracrRNA and guides the cas protein to shear double-stranded DNA. Traditional methods of sgRNA synthesis include plasmid transfection, in vitro transcription, etc. but suffer from low efficiency and high cytotoxicity, whereas the synthesis of sgRNA by chemical methods can effectively avoid the above problems.
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Service Advantages

Synthesized lengths of 93-150 nt guarantee 100% sequence correctness

Synthesized lengths of 93-150 nt guarantee 100% sequence correctness

01/03
The stability and editing efficiency of sgRNAs can be significantly improved by 2'-methoxy modification, sulfur modification, and so on

The stability and editing efficiency of sgRNAs can be significantly improved by 2'-methoxy modification, sulfur modification, and so on

02/03
With simple and fast, low off-target effect, high editing efficiency, high transfection efficiency, no cytotoxicity, no DNA interference, etc.

With simple and fast, low off-target effect, high editing efficiency, high transfection efficiency, no cytotoxicity, no DNA interference, etc.

03/03

Service Period

Special modifications Base length (nt) Method of purification Periods
3 thio and methoxy modifications at each of the 3' and 5' ends 60-130 DSL/HPLC 5-10 working days

 

Service Process

Sample Acceptance

Sample Acceptance

synthesize

synthesize

ammonia liberation process

ammonia liberation process

Remove the 2-bit protection base

Remove the 2-bit protection base

purify

purify

quarantine

quarantine

packaged and dried

packaged and dried

send out goods

send out goods

Delivery Results

If you have other requests, send an e-mail to marketing@gentegen.com,We will be at your service as soon as possible.

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Inquiry and Ordering

Inquiry and Ordering

Directly log in the “GBS Online Ordering System”, automatically get a quote and place an order; download the corresponding service order form in the “Download” area, send an e-mail, we will provide you with a quote as soon as possible.

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Ordering Information

Ordering Information

Email:marketing@gentlegen.com Tel:0512-67998818 ext. 8888

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