Whole genome resequencing of animals and plants

Whole genome resequencing is the process of sequencing the DNA of a species with a known genome sequence and conducting individual or population analysis based on it. Whole genome resequencing can detect a large amount of variation information through sequence alignment, including single nucleotide polymorphisms (SNPs), insertions and deletions (InDel), structural variations (SV), and copy number variations (CNV). Based on the detected variations, further research can be conducted on the species characteristics, population evolution issues, and target trait gene loci of animals and plants. With the reduction of sequencing costs and the increase of known genome sequence species, whole genome resequencing has become one of the most rapid and effective methods in molecular breeding and population evolution of animals and plants. The use of whole genome resequencing technology can help quickly discover genetic variations related to important traits in animals and plants, and be applied in molecular breeding to shorten the breeding cycle

Service Advantages

Simple technology, good stability, and high-quality (Q30) sequencing data

01/04

Rich detection of variant types: can detect multiple variant types such as SNP, InDel, SV, and CNV, and can be used as molecular markers

02/04

High density markers: capable of detecting SNP information across the entire genome, while also detecting low-frequency SNPs

03/04

High cost-effectiveness: Compared with whole genome de novo sequencing, it takes less time and costs less

04/04

Service Period

Sample type	Conventional DNA small fragment library	PCR free library
DNA sample	DNA≥ 1 μ g	DNA≥ 3 μ g
Fresh cultured cells (number of cells)	≥ 5× 106 cell	≥ 1× 107 cell
Animal tissue	≥ 300mg	≥ 500mg
Plant tissue	≥ 1g	≥ 2g
Whole blood	≥ 1 mL	≥ 2 mL

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Service Process

experimental design

nucleic acid extraction

library construction

High throughput sequencing

Bioinformatics analysis

Delivery Results

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raw data

analysis report

Service&Support

Inquiry and ordering

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