The CRISPR-Cas9 system is a powerful tool for gene editing, capable of editing almost any gene by designing guide RNAs with different sequences to target DNA sequences. sgRNA library screening involves the use of CRISPR/Cas9 technology to establish genome-wide knockout libraries of target species or knockout libraries related to specific functions, and then discovering genes relevant to the screened phenotype through functional screening and enrichment as well as subsequent Through functional screening and enrichment, followed by PCR amplification and in-depth sequencing analysis, genes related to the screening phenotype are discovered.

GentleGen has standardized genome-wide knockout libraries for both human and mouse, which is convenient for large-scale genome-wide screening. In addition, we also provide customized gRNA libraries, which are designed and synthesized to target specific pathways or genes according to customers' needs.