No degradation, lyophilized primers can be stored stably at room temperature for at least 2 weeks. The normal shipping time is usually 1-3 days, so the primers you receive will not degrade.

After the primers are synthesized, they undergo a series of processing and purification steps and are spin-dried into tablets. Unsolubilized primers are very stable and can be stored at -20°C for 2-3 years or even longer. Dissolved primers can be stored for at least half a year at -20°C avoiding repeated freezing and thawing. If the reproducibility of the experiment is required to be higher, the synthesized OD value is larger or the synthesized amount of single primer synthesis is larger, it is recommended to dilute the dissolved primer into 100M storage solution beforehand, and store it in several portions in the refrigerator at -20C. Before use, dilute the concentrated solution into a working solution (10-20uM) and carry out the experiment. Fluorescence modification primers need to be stored away from light.

The dried primer material is very loose, and before opening the cap to dissolve it is best to centrifuge for 1 minute at 3,000-4,000 rpm or tap the tube vertically upwards on a tabletop a few times to collect the primer powder into the bottom of the tube and prevent the primer from dispersing when the cap is opened.
Add deionized sterile water or 10 mMTris pH 7.5 buffer according to the calculated volume, leave at room temperature for a few minutes, mix up and down with shaking, and centrifuge to collect the solution to the bottom of the tube. The water used to dissolve the primers should generally not be distilled because some distilled water has a low pH (pH 4-5) and the primers are unstable under these conditions. Our synthesis report form gives the amount of water to be added per tube of primer diluted to a concentration of 100 uM (i.e., 100 pmopl), and you can add an appropriate amount of nuclease-free double-distilled water (pH > 6.0) or TE buffer (pH 7.5-8.0) to suit your experimental needs.