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Stabilized cell line construction

Stable transduced cell lines, i.e., stably expressed cell lines, are cell lines constructed based on a particular cell line that consistently overexpresses or interferes with a specific gene. By cloning an exogenous DNA/shRNA fragment into a specific vector containing a resistance gene, the recombinant vector is integrated into the chromosome of the host cell after infection, and is stably expressed and passed on as the cell proliferates, and then the stable-transformed cell line can be obtained by resistance screening.
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Service Advantages

High-quality platform: a mature and stable platform for screening stable cell lines with diversified technologies

High-quality platform: a mature and stable platform for screening stable cell lines with diversified technologies

01/03
Cost-effective: using the carrier method, short process, low cost

Cost-effective: using the carrier method, short process, low cost

02/03
High Expression Efficiency and Phenotypic Stability: Ensure high efficiency and stable expression for more than 20 generations.

High Expression Efficiency and Phenotypic Stability: Ensure high efficiency and stable expression for more than 20 generations.

03/03

Service Period

Service Description Service Description Completion Cycle (Working Days)
Overexpression of stabilized cell lines Low Difficulty Cells + Medium Difficulty Cells 5-8 weeks
Difficult Cells 6-10 weeks
Interference Stabilization Cell Line Low Difficulty Cells + Medium Difficulty Cells 5-8 weeks
Difficult Cells 6-10 weeks

Service Process

vector construction

vector construction

plasmid extraction

plasmid extraction

transfected cells

transfected cells

isolation culture

isolation culture

stable transducer cell line

stable transducer cell line

Delivery Results

If you have any other requirements, please send an email to marketing@gentlegen.com,We will serve you as soon as possible.

Overexpression/interference with stabilized turnover monoclonal 1+control cell line

Validation of mRNA levels

Lab Report

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Inquiry and Ordering

Inquiry and Ordering

Directly log in the “GBS Online Ordering System”, automatically get a quote and place an order; download the corresponding service order form in the “Download” area, send an e-mail, we will provide you with a quote as soon as possible.

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Ordering Information

Ordering Information

Email:marketing@gentlegen.com Tel:0512-67998818 ext. 8888

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