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Recombinant Protein Expression Service

Recombinant protein expression service

Recombinant proteins are widely used in life sciences and biomedical fields, with the advantages of large yield, high stability and flexible design, etc. However, the long experimental period and many experimental links of recombinant protein expression purification have hindered the protein research process.

GentleGen has a one-stop service platform from gene synthesis to recombinant protein expression and purification, which can provide codon optimization, expression vector design, optimization of expression conditions and other services, to help customers solve the recombinant protein expression and purification of all aspects of the problem, to help scientific research and drug discovery.
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Type of service Services Service Description Service interval Delivery standards
E. coli Expression Service Construction of expression vectors Codon Optimization & Gene Synthesis 10 days One-step affinity purification
SDS-PAGE>80%
COA Report
Expression characterization, solubility assay Transformation of expression strains for SDS-PAGE gel expression assay
Different temperature incubation allows for solubility testing
Optimization of different induction conditions, SDS-PAGE and WB conventional labeled antibody detection
7 days
Amplified expression 1L, protein purification, delivered protein >1mg Above expression validation steps + 1L bacterial solution one-step affinity purification 10 days
Mammalian Cell Expression Service Gene synthesis and plasmid macro pumping Codon optimization and expression vector construction, plasmid macro-drawing and plasmid validation 10-15 days Small test expression results analysis report SDS-PAGE analysis report WB results
Express oneself in a small way Transient transfection of mammalian cells, one-step affinity purification, QC assay
Amplified expression (1-50L) Amplified expression, protein purification, QC assay 10-15 days Purified protein (SDS-PAGE>90%, endotoxicity <1EU/mg)
Shipment report, SEC-HPLC
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Service Process

Gene synthesis

Gene synthesis

Expression of vector constructs

Expression of vector constructs

Expression verification

Expression verification

Protein purification

Protein purification

Quality control

Quality control

Cold chain shipping

Cold chain shipping

Service&Support

Inquiry and Ordering

Inquiry and Ordering

Login directly to GentleGen GBSOnline Ordering System,Automatically obtain quotes and place orders. Download the corresponding service order form in the“Downloads”section, send an e-mail and we will provide you with a quotation as soon as possible.


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Ordering Information

Ordering Information

Email.marketing@gentlegen.com Tel:0512-67998818 ext. 8888


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