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Recombinant Protein Expression Service
Recombinant protein expression service
Type of service | Services | Service Description | Service interval | Delivery standards |
---|---|---|---|---|
E. coli Expression Service | Construction of expression vectors | Codon Optimization & Gene Synthesis | 10 days | One-step affinity purification SDS-PAGE>80% COA Report |
Expression characterization, solubility assay | Transformation of expression strains for SDS-PAGE gel expression assay Different temperature incubation allows for solubility testing Optimization of different induction conditions, SDS-PAGE and WB conventional labeled antibody detection |
7 days | ||
Amplified expression 1L, protein purification, delivered protein >1mg | Above expression validation steps + 1L bacterial solution one-step affinity purification | 10 days | ||
Mammalian Cell Expression Service | Gene synthesis and plasmid macro pumping | Codon optimization and expression vector construction, plasmid macro-drawing and plasmid validation | 10-15 days | Small test expression results analysis report SDS-PAGE analysis report WB results |
Express oneself in a small way | Transient transfection of mammalian cells, one-step affinity purification, QC assay | |||
Amplified expression (1-50L) | Amplified expression, protein purification, QC assay | 10-15 days | Purified protein (SDS-PAGE>90%, endotoxicity <1EU/mg) Shipment report, SEC-HPLC |
Service Process
Gene synthesis
Expression of vector constructs
Expression verification
Protein purification
Quality control
Cold chain shipping
Service Advantages
Wide range of expression vectors and strains
Optimization of expression conditions to improve protein expression
Rapid gene synthesis platform
Intelligent production platform
Application Area
Service&Support
Inquiry and Ordering
Login directly to GentleGen GBSOnline Ordering System,Automatically obtain quotes and place orders. Download the corresponding service order form in the“Downloads”section, send an e-mail and we will provide you with a quotation as soon as possible.
If you have any questions, please contact us