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AbTaq DNA Polymerase

AbTaq DNA Polymerase

Price:

¥1200
58 Integral

Number:

004174

Specifications:

500U

Product details

Product Introduction

AbTaq DNA Polymerase is a hot-start Taq DNA Polymerase modified with a monoclonal antibody, which effectively seals off the DNA polymerase activity below 55°C, and irreversibly dissociates at higher temperatures, restoring the polymerase activity, thus avoiding non-specific amplification at low temperatures. This product is mainly used for fluorescence quantitative PCR, especially Taqman probe method.

Product Components

 

Groups Size
AbTaq DNA Polymerase (5 U/µl)
100 µl
10× Taq Reaction Buffer
3×1 ml

 

Storage Condition

-20°C for storage.

Quality Control

Nucleic acid endonuclease activity assay:25W 2xTaG-AS PCR MIx/2xTaG-AS PCR MIx(+Dye) or 10u 5xTaG-AS PCR MIx/5xTaG-AS PCR MI(+Dye) were formulated into a 50-reaction system with 200 ng of super-helical plasmid DNA, and after co-incubation for 4 h at 37°C, less than 10% of the plasmid DNA was transformed into a deletion or linear state.


Non-specific nuclease activity assay:25uI 2xTaG-AS PCR MIx/2xTaG-AS PCR MIx(+Dye) or 10uI 5xTaG-AS PCR MI/5xTa-AS PCR Mix(+Dye) was prepared with 15ng double-stranded DNA fragments to form a 50-reaction system, and the reaction was incubated at 37°C for 16h, and no change of double-stranded DNA substrate was detected using agarose gel electrophoresis. The double-stranded DNA substrate was detected by agarose gel electrophoresis without any change.

 

Host DNA residue detection:
Using E. coli 16S rDNA specific primer probe set, using fluorescence quantitative PCR method to detect 5 U AbTaq DNA Polymerase, E. coli host genome DNA residue is less than 1 copy.

Usage

see instruction manual

Contact Information

Customer Service Hotline:

+86 512 6799 8818

Company email:

marketing@gentlegen.com

Company address:

No.111, Wusongjiang Avenue, Guoxiang Street, Wuzhong District, Suzhou, China

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