The concentration of TaG-ASPCR MIx /TaG-AS PCRMi(+Dye) is 2x/5x, which is convenient and quick to use, and can reduce the contamination in the process of PCR operation. When using, just take an appropriate amount of 2x/5xTaG-AS PCR or 2x/5xTaG-AS PCR MIx(+Dve) to add templates and primers, and add ddH20 to make up the volume so that the The PCR reaction can be carried out by adding the template and primer to the reaction system at a concentration of 1x. The PCR product with a prominent A base at the 3' end can be used directly for TIA cloning after purification. The Tag DNA polymerase in the Mx is a Taq-AS (Advancedstrong) DNAPolymerase mutant obtained through screening, which is able to efficiently amplify DNA fragments of ≤5kb with good inhibitor tolerance, and its amplification speed is about 3 times that of the ordinary Taq DNA polymerase, which can greatly reduce the time required for PCR extension and shorten the whole PCR reaction. This can greatly reduce the time needed for PCR extension and shorten the whole PCR reaction. Unlike the fast Tq polymerase of the fusion protein principle, Tag-AS is closer to WT-Taq, and is less prone to electrophoretic band dispersion, band dragging, or fragment size changes.
TaG-ASPCR MI(+Dye)(2x)/(5x) contains two types of dyes, PCR products can be directly spotted without adding Loading Bufer, and two indicator bands, purple and yellow, will appear during electrophoresis. This dye does not affect the efficiency of PCR amplification, but it is recommended that PCR products be purified prior to analysis for optical analysis such as absorbance and fluorescence.