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Sanger sequencing service

Sanger sequencing service

Why are the sequencing results not the sequence I need?

We can not judge whether the sequences measured are consistent with your expected results, if there is any doubt about the results, we can carry out re-sequencing to verify, if the two results are inconsistent, the first time is free of charge, if the two results are consistent, then you need to charge the cost of two sequencing.

Why does the sequencing peak plot result in a decaying trend after 300-400bp?

There are two types of sequencing peak map analysis modes, and we selected the sequencing peak map analysis mode to retain the real sequencing peak map without flattening or any beautification.

Why must fragment lengths be filled in for PCR samples?

Because PCR sample sequencing we need to identify whether the band size meets your expectations, if not, it is likely that the amplified band is not your target band, resulting in sequencing does not meet expectations, and identification of the existence of multiple bands we can also be based on the length of the fragments provided by you to cut the gel recovery of your requested band for sequencing.

Why is the sequencing result positive when the synthesized primer information is filled in and reverse sequencing is told?

Sequencing is based on the primer sequence provided by the teacher to synthesize and sequencing, sequencing can not control the primer direction are based on the primer direction extension sequencing, such as synthesis of primers need to reverse sequencing needs to be the teacher to carry out their own reverse complementary, or to send an order to inform the sequence of the need to carry out the reverse complementary we will arrange for you to reverse complementary and then synthesize and sequencing.

Why did the sequencing fill out the sequencing pass 3k and the sequencing result was only about 1k?

Normal a sequencing results to ensure the accuracy of about 800bp, long fragment sequencing requires multiple reaction sequencing results splicing in order to pass, such as the order sequencing requirements fill in the pass we will be based on the results of the sequencing results have been designed to extend to the pass and after the pass to send you the results of the splicing, such as the process of passing the test in the process of the problem will be timely feedback to you.
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