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NGS CRISPR therapy safety testing

NGS CRISPR therapy safety testing

In recent years, the field of Cell&Gene Therapy (CGT) has developed rapidly, with significant breakthroughs in AAV, CAR-T, CRISPR, and RNA drugs. Among them, gene editing therapy with CRISPR technology as the core is particularly prominent. Gene editing is an emerging genetic engineering technology that can precisely modify specific target genes in an organism's genome.

In order to accelerate the verification speed of off target site detection of gene editing drugs and improve detection efficiency, GentleGen has independently developed a fully automated PCR platform, which can achieve the full process automation of thousands of sites from templates to products in one day, greatly accelerating the safety evaluation of gene editing. Combined with the high-throughput NGS sequencing platform, it provides powerful technical support for off target evaluation of gene edited drugs.
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Service Advantages

Highly automated: The process from sample to PCR product is automated without the need for manual intervention

Highly automated: The process from sample to PCR product is automated without the need for manual intervention

01/04
Efficient and cost-effective: Based on an automated PCR platform, thousands of samples can be efficiently and cost effectively PCR and purified daily

Efficient and cost-effective: Based on an automated PCR platform, thousands of samples can be efficiently and cost effectively PCR and purified daily

02/04
Accuracy: Accurately and independently control the reaction conditions of different samples to ensure the reliability of PCR results

Accuracy: Accurately and independently control the reaction conditions of different samples to ensure the reliability of PCR results

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Stability: 100% PCR consistency, the platform has undergone stability testing and iteration on hundreds of thousands of samples

Stability: 100% PCR consistency, the platform has undergone stability testing and iteration on hundreds of thousands of samples

04/04

Service Period

Sample type Sample requirements Detection method Completion cycle (working days)
Gene editing efficiency detection - amplicon sequencing 1. Provide PCR products or genomic DNA; 2. The amount of genome provided is determined by the number of loci that need to be detected Second generation sequencing Evaluate based on specific project
Off target validation of gene editing - high-throughput amplification sequencing Second generation sequencing

Service Process

Sample reception

Sample reception

Sample quality inspection

Sample quality inspection

Library preparation

Library preparation

Library sequencing

Library sequencing

Issue a report

Issue a report

Delivery Results

If you have any other requirements, please send an email to order@gentlegen.com,We will serve you as soon as possible.

Original sequencing results

Analysis and Testing Report

Service&Support

Inquiry and Ordering

Inquiry and Ordering

Directly log in to GentleGen GBS Online Ordering System,Automatically obtain quotes and place orders; stay“Data Download”Download the corresponding service subscription form and send an email. We will provide you with a quotation as soon as possible.

Download

Download

Product manual download、order form

Ordering Information

Ordering Information

Email:marketing@gentlegen.com Phone: 0512-67998818, ext. 8888

Sample Preparation

Provide PCR products or genomic DNA

Provide PCR products or genomic DNA

The amount of genome provided is determined by the number of loci that need to be detected

The amount of genome provided is determined by the number of loci that need to be detected

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