Solving the pain points of RNAi experiments: GentleGen's shRNA "Three-for-One" service, say goodbye to "mystical" knock-down methods, and obtain guaranteed shRNA!

Release time:2026-06-25

In RNA interference experiments, the most troublesome aspect is the "low sequence efficacy rate." The three shRNAs painstakingly designed and synthesized had knockdown efficiency below 50%, making the experiment impossible to advance and unable to determine whether it was a carrier issue, sequence issue, or operational issue.

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To address this core pain point, GentleGen leverages its powerful design and synthesis platform to launch the shRNA "Three-for-One" assurance service, letting data speak and taking responsibility for results.

GentleGen standardizes service processes

Clients provide: target gene ID, species, or sequences

Services: shRNA design + vector construction

Delivery content: 3 target shRNA + 1 negative control (NC) + COA file

Service period: 5-10 working days

GentleGen's core commitment

GentleGen Promises: Under the strict premise of confirming transfection efficiency greater than 85%, at least one of the three shRNAs we deliver can achieve a knockdown efficiency of over 70% at the mRNA level. If this condition is not met, GentleGen will design and synthesize a new shRNA for you free of charge.

GentleGen's shRNA service advantages

Professional bioinformatics design: Target locus sequences are designed for specific genes, with 2-3 genes per gene, ensuring that at least one succeeds.

High success rate: The independently developed fast and efficient shRNA cloning method is unaffected by hairclip structure, resulting in a very high success rate.

100% sequence accuracy: The synthesized shRNA plasmid is verified by sequencing to ensure 100% sequence accuracy and provide reliable sequencing results.

GentleGen meets advanced needs all in one place

Additionally, for teachers needing stable strain construction or animal experiments, GentleGen simultaneously offers shRNA lentiviral packaging "Three-for-One Knockdown Guarantee" service.

The control virus showed exactly the same EGFP fluorescence and Puro resistance as the target virus;
Virus titer is stable≥ 1E+8 TU/mL, 3+1ml;
Promises that when transfection efficiency ≥ 90%, at least one mRNA interference will be ≥ 70% efficiency

GentleGen always insists on technological innovation as the driving force and customer-centric approach. For detailed information about related services, please send an email to marketing@gentlegen.com.

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